FITC-Dextran (MW 40000) is a fluorescent probe for fluorescein isothiocyanate (FITC) dextran (Ex=495 nm; Em=525 nm). FITC-Dextran (MW 40000) can be used as a marker to reveal heat shock-induced cell damage and to study the early and late stages of apoptosis. FITC-Dextran (MW 40000) can also be used for cell permeability studies, such as blood-brain barrier permeability and determination of the extent of blood-brain barrier disruption^[1][2][3].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).

细胞标记^[1]:
适用于正在发生凋亡的 HeLa 细胞和人外周血单个核细胞 (PBMC) (活的 HeLa 和 PBMC 不被 FITC-Dextran 染色)。
1. 43.5°C 孵育 1 h，37°C 孵育 8 h，诱导细胞凋亡。
2. 将细胞悬浮在 100 μL 的培养基中，在 Q-prep 管与 10 μL 的 propidium iodide (PI)，10 μL 的 FITC-Dextran (MW 40000) 混合，PI 和 FITC-Dextran (MW 40000) 的最终浓度分别为 7.5 μM 和 1.13 μM。
3. 将细胞在室温黑暗条件下培养 25 分钟。
4. 取 3 mL 培养基的标记细胞，500 g 离心 10 分钟。
5. 取离心细胞，加入 1 mL 培养基，用流式细胞术或荧光显微镜分析 (PI: Ex=500 nm, Em=600 nm; FITC-Dextran (MW 40000): Ex=495 nm, Em=525 nm)。


细胞旁渗透性检测^[4]
1. 在 transwell 室的基础培养基中加入 FITC-Dextran (0.1 mg/mL)。
2. 在 15 分钟后从 transwell 插入器中收集培养基。
3. 测量荧光信号 (Ex=485 nm, Em=538 nm)。
4. 根据荧光强度计算 FITC-Dextran 浓度。
5. 计算渗透率。

Tanda has not independently confirmed the accuracy of these methods. They are for reference only.

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).

肠屏障功能测定^[5]
1. 小鼠饥饿处理 4 小时。
2. FITC-Dextran MW 40000 灌胃小鼠 (0.6 mg/g)。
3. 在 4 h 内测量荧光强度 (Ex nm/Em 520 nm)。

Tanda has not independently confirmed the accuracy of these methods. They are for reference only.

40000(Average)

Solid

60842-46-8

525

488

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)