Pyranine (HPTS; Solvent Green 7) is a pH-sensitive fluorescent indicator. Pyranine acts as a class of fluorescent chemosensor for the Cu⁺ ion(λex=450 nm, λem=510 nm)^[1][2][3].

Pyranine is capable of discriminating ranges of cations from the Cu⁺ ion, even in competing environment. Pyranine displays a rapid fluorescence response (t_1/2=1.66 min) towards the Cu⁺ ion, and the micromolar detection limit enables the detection of the ion in environmental samples. The observed stoichiometry of complexation between Pyranine and Cu⁺ is 2:1^[1].
The pH-sensitive fluorescent indicator Pyranine is studied to determine its usefulness as probes for the living cornea and anterior chamber. Adequate concentrations are reached in the cornea and anterior chamber after topical administration; measurements can be made by fluorophotometry for many hours. The pH is calculated by measuring the ratio of fluorescent intensity at two excitation wavelengths, I463/I404, a measurement which is dependent on pH but independent of the concentration of the fluorophore and other variables which can alter the intensity. In the rabbit eye, Pyranine in the cornea and anterior chamber is observed to undergo easily measurable changes in fluorescent ratios associated with lid closure and contact lens wear, indicating its sensitivity to mild changes in pH^[2].

Tanda has not independently confirmed the accuracy of these methods. They are for reference only.

524.39

Solid

C₁₆H₇Na₃O₁₀S₃

6358-69-6

510

450

溶剂绿7

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。