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5-FAM (Synonyms: 5-羧基荧光素; 5-Carboxyfluorescein)
- 目录号: TD-66022 纯度: 99.56%
| 生物活性 | 5-FAM (5-Carboxyfluorescein) is a green fluorescent reagent used for in situ labeling peptides, proteins and nucleotides. 5-FAM is a single isomer with Ex/Em of 490 nm/520 nm[1]. | ||||||||||||||||
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| 体外研究 (In Vitro) | 5-FAM contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules. In addition to its relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility, fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser[1]. Tanda has not independently confirmed the accuracy of these methods. They are for reference only. | ||||||||||||||||
| 分子量 | 376.32 | ||||||||||||||||
| 性状 | Solid | ||||||||||||||||
| Formula | C21H12O7 | ||||||||||||||||
| CAS 号 | 76823-03-5 | ||||||||||||||||
| Emission (Em) | 525 | ||||||||||||||||
| Excitation (Ex) | 488 | ||||||||||||||||
| 中文名称 | 5-羧基荧光素 | ||||||||||||||||
| 运输条件 | Room temperature in continental US; may vary elsewhere. | ||||||||||||||||
| 储存方式 | 4°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light) | ||||||||||||||||
| 溶解性数据 | In Vitro: DMSO : ≥ 31 mg/mL (82.38 mM) H2O : 1 mg/mL (2.66 mM; ultrasonic and warming and heat to 80°C) * "≥" means soluble, but saturation unknown. 配制储备液
* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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