Biotin NHS is an amino reactive biotin reagent used in the preparation of biotinylated surfaces or polypeptides.

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
? Calculations:
? For best results, use ≥ 12-fold molar excess of biotin for a 10 mg/mL protein solution or ≥ 20-fold molar excess of biotin for a 2 mg/mL protein solution.
? 1.Calculate millimoles of biotin reagent to add to the reaction for a 20-fold molar excess:
? mL protein × (mg protein/mL protein) × (mmoL protein/mg protein) × (20 mmoL protein/mmoL protein) = mmoL Biotin.
? * 20 = Recommended molar fold excess of biotin for 2 mg/ml protein sample
? 2. Calculate microliters of 10 mM biotin reagent solution (prepared in Step B.3) to add to the reaction:
? mmoL Biotin × (1000000 μL/L) × (L/ 10 mmoL) =μL Biotin
? Biotin Labeling Reaction:
? 1. Dissolve 1-10 mg protein in 0.5-2.0 mL PBS according to the calculation made in Calculations.
? 2. Before use, prepare a 10 mM solution of the biotin reagent in an organic solvent such as DMSO.
? 3. Add the appropriate volume (μL) of 10 mM biotin reagent solution to the protein solution.
? 4. Incubate reaction on ice for two hours or at room temperature for 30 minutes.
? 5. To tests the labeled protein by ELISA or Western blot.

Tanda has not independently confirmed the accuracy of these methods. They are for reference only.

341.38

Solid

C₁₄H₁₉N₃O₅S

35013-72-0

生物素N-羟基琥珀酰亚胺酯

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture and light

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请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液；该产品在溶液状态不稳定，建议您现用现配，即刻使用。