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G蛋白突变抗体

RAS(G13V) [26477]
[100ul]


Catalog Number:  26477
Gene Symbol:  H-Ras; K-Ras; N-Ras
Description:   Anti RAS(G13V) Mouse Monoclonal Antibody
Background:   Ras protein is a membrane-associated GTPase signaling protein that regulates proliferation, differentiation, and cell survival. Ras G13V mutation result in decreased GTPase activity and constitutive signaling, and is correlated with the development of pancreatic intraepithelial neoplasia and lung lesions.
Immunogen:   A synthetic peptide from the internal region of Ras which includes the mutation of G13V, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:500-1:1000 
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes G13V mutant, but not wild-type RAS of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant Ras proteins. Purified His-tagged RAS(G13V) protein (lane 1), corresponding wildtype protein (lane 2), His-tagged Ras(G13S) protein (lane 3), His-tagged Ras(G13C) protein (lane 4), His-tagged Ras(G13R) protein (lane 5), His-tagged Ras(G13A) protein (lane 6) were blotted with Anti RAS(G13V) monoclonal antibody (Cat. #26477).
Immunofluorescence:  
Immunofluorescence of cells expressing Ras proteins with Anti RAS(G13V) antibody. HEK293T cells were transfected with pCDNA3-GFP-RAS(G13V) plasmid ,pCDNA3-GFP Ras(WT) plasmid, pCDNA3-GFP Ras(G13S) plasmid, pCDNA3-GFP Ras(G13C) plasmid, pCDNA3-GFP Ras(G13R) plasmid, pCDNA3-GFP Ras(G13A) plasmid or pCDNA3-GFP Ras(G13D) plasmid, then fixed and stained with Anti RAS(G13V) monoclonal antibody (Cat. #26477).



Publications:
1.  DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model
    Gene Therapy 21, 888-896 (October 2014)
2.  Are all RAS mutations the same? Coexisting KRAS and NRAS mutations in a caecal adenocarcinoma and contiguous tubulovillous adenoma
    J Clin Pathol. 2015 Aug;68(8):657-60. doi: 10.1136

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