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PIK3CA(E545K) [21145]
[100 µL]
| Catalog Number: 21145 |
| Gene Symbol: PIK3CA(E545K) |
| Description: Anti PIK3CA(E545K) Rabbit Polyclonal Antibody |
| Background: Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The catalytic subunit is encoded by the PIK3CA gene. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2. The PIK3CA gene is one of the most frequently mutated genes in human cancers. |
| Immunogen: A synthetic peptide from the internal region of PIK3CA(E545K) of human origin. |
| Applications: ELISA, WB, IHC, IP |
| Recommended Dilutions: ELISA: 1:100-1:5000 WB: 1:100-1:1000 IHC: 1:10-1:100 |
| Concentration: 0.2 mg/ml |
| Host: Rabbit |
| Format: Liquid |
| Clonality: Polyclonal |
| Purity: Purified from serum |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti PIK3CA(E545K) antibody recognizes PIK3CA(E545K) of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Western blot: |
 | Western blot analysis of PIK3CA wild type and E545K proteins. Equal amounts of purified PIK3CA(E545K)(amino acids 461-640) and corresponding wild type proteins were loaded and blotted with Anti PIK3CA(E545K) rabbit polyclonal antibody (Cat. # 21145). |
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