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Active Rab4A GTP [26921]
[30 µL]
| Catalog Number: 26921 |
| Gene Symbol: RAB4 |
| Description: Anti Active Rab4 Mouse Monoclonal Antibody |
| Background: Rab4 belongs to the small GTPase Rab family. It interacts with RAB11FIP1, RABEP1, ZFYVE20, RUFY1, SGSM1, SGSM2 and SGSM3. It participates in protein transport and in vesicular traffic. |
| Immunogen: Recombinant full length protein of active Rab4A. |
| Applications: IP, IHC |
| Recommended Dilutions: 1 μg for 1~2 mg total cellular proteins for IP 1:50~100 dilution for IHC |
| Concentration: 1 mg/ml |
| Host: mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti-active Rab4 antibody recognizes active Rab4 from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Immunoprecipitation/Western blot: |
 | Rab4A activation assay. Purified GST-tagged Rab4A proteins (Cat. #10130) were immunoprecipitated with the anti active Rab4 monoclonal antibody (Cat. No. 26921) after treated with GDP (lane 1) or GTPγ S(lane 2), and was blotted with anti Rab4A polyclonal antibody (Cat. No. 21067). Input control is shown in the bottom panel. |
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