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Active Rab5 GTP [26911]
[30 µL]
| Catalog Number: 26911 |
| Gene Symbol: RAB5 |
| Description: Anti Active Rab5 Mouse Monoclonal Antibody |
| Background: Rab5 is a member of Rab-family GTPases. Rab5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway. |
| Immunogen: Recombinant full length protein of active Gao |
| Applications: IP, IHC |
| Recommended Dilutions: 1 µg for ~2 mg total cellular proteins for IP 1:100 dilution for IHC |
| Concentration: 1 mg/ml |
| Host: mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG1 |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti-active Rab5 antibody recognizes active Rab5 proteins of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
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| Immunoprecipitation/Western blot: |
 | Rab5 activation assay. Purified full-length Rab5A proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti active Rab5 monoclonal antibody (Cat. No. 26911). Immunoblot was with an anti Rab5 polyclonal antibody (Cat. No. 21151). |
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