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Active Ran-GTP [26915]
[30 µL]
| Catalog Number: 26915 |
| Gene Symbol: RAN |
| Description: Anti Active Ran Mouse Monoclonal Antibody |
| Background: Ran (Ras-related nuclear protein) is a member of the Ras-superfamily GTPases. Ran is invovled in the transport of proteins across the nuclear envelope, as well as in microtubule organization during mitosis. |
| Immunogen: Recombinant full length protein of active Ran. |
| Applications: IP, IHC |
| Recommended Dilutions: 1 µg for 1~2 mg total cellular proteins for IP; 1:100 dilution for IHC |
| Concentration: 1 mg/ml |
| Host: mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti-active Ran antibody recognizes active Ran of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Immunoprecipitation/Western blot: |
 | Ran activation assay. Purified Ran proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti-active Ran monoclonal antibody (Cat. No. 26915). Immunoblot was with an anti-Ran polyclonal antibody. |
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