欢迎来到武汉天德生物科技有限公司官方网站！

设为首页 / 收藏本站 / 关于我们

请拨打服务热线：

027-87561633

产品中心PRODUCT CENTER

全国热线：027-87561633

当前位置：首页 > 产品中心 > 阿尔茨海默症相关产品 > Aβ相关产品

Aβ相关产品

测试测试

概述

  • 产品名称

    Anti-Presenilin 2/AD5抗体[EP1515Y]
    参阅全部 Presenilin 2/AD5 一抗

  • 描述

    兔单克隆抗体[EP1515Y] to Presenilin 2/AD5

  • 宿主

    Rabbit

  • 特异性

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • 经测试应用

    适用于: Flow Cyt (Intra), ICC, WB, IHC-P, IPmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide within Human Presenilin 2/AD5 aa 300-400 (C terminal). The exact sequence is proprietary.

  • 阳性对照

    • WB: HeLa, HepG2, C2C12, C6, and Neuro-2a lysates; IHC-P: Human cerebrum tissue; ICC: PC-12 cells; Flow Cyt (intra): HeLa cells; IP: HeLa and PC-12 cell lysates.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • 浓度
    • 100 µl 浓度为 0.55 mg/ml
    • 40 µl 浓度为 0.55 mg/ml
    • 10 µl 浓度为 0.55 mg/ml

    <div class="concentration-holder" data-hcaptchasitekey="193441f7-8cd9-40a4-8075-4640966eb995" "="" style="border: 1px solid rgb(222, 223, 223); font-family: inherit; font-style: inherit; font-weight: inherit; margin: 0px 0px 1em; outline: none 0px; padding: 10px; vertical-align: baseline; box-sizing: border-box; bottom: 5px; clear: both; width: 400px;">


  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EP1515Y

  • 同种型

    IgG

  • 研究领域

    • Neuroscience
      •  
    • Neurology process
      •  
    • Notch Pathway
    • Neuroscience
      •  
    • Neurology process
      •  
    • Neurodegenerative disease
      •  
    • Alzheimer's disease
      •  
    • Proteases
    • Cancer
      •  
    • Cancer Metabolism
      •  
    • Response to hypoxia
    • Metabolism
      •  
    • Pathways and Processes
      •  
    • Metabolism processes
      •  
    • Hypoxia
    • Metabolism
      •  
    • Types of disease
      •  
    • Neurodegenerative disease
    • Metabolism
      •  
    • Types of disease
      •  
    • Cancer

    • 相关产品

    • Alternative Versions

      • Anti-Presenilin 2/AD5 antibody [EP1515Y] - BSA and Azide free (ab239841)

    • Isotype control

      • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab51249于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用Ab评论说明
    Flow Cyt (Intra) 
    1/50.
    ICC(1)
    1/50 - 1/250.
    WB(4)
    1/20000. Detects a band of approximately 23 kDa (predicted molecular weight: 50 kDa).
    IHC-P(1)
    1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    See IHC antigen retrieval protocols.

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

    Read More
    IP(1)
    1/20.

    靶标

    • 功能

      Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. May function in the cytoplasmic partitioning of proteins.

    • 组织特异性

      Isoform 1 is seen in the placenta, skeletal muscle and heart while isoform 2 is seen in the heart, brain, placenta, liver, skeletal muscle and kidney.

    • 疾病相关

      Defects in PSEN2 are the cause of Alzheimer disease type 4 (AD4) [MIM:606889]. AD is an autosomal dominant Alzheimer disease. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
      Defects in PSEN2 are the cause of cardiomyopathy dilated type 1V (CMD1V) [MIM:613697]. It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.

    • 序列相似性

      Belongs to the peptidase A22A family.

    • 结构域

      The PAL motif is required for normal active site conformation.

    • 翻译后修饰

      Heterogeneous proteolytic processing generates N-terminal and C-terminal fragments.
      Phosphorylated on serine residues.

    • 细胞定位

      Endoplasmic reticulum membrane. Golgi apparatus membrane.

    • 别名

      • AD3L antibody
      • AD3LP antibody
      • AD4 antibody
      see all

    图片

    • Western blot - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)
      Western blot - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)
      All lanes : Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249) at 1/20000 dilution (Purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
      Lane 3 : C2C12 (Mouse myoblasts myoblast) whole cell lysates
      Lane 4 : C6 (Rat glial tumor glial cell) whole cell lysates
      Lane 5 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 50 kDa
      Observed band size: 23 kDawhy is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling Presenilin 2/AD5 with purified ab51249 at 1/500 dilution (0.51 µg/ml). Heat mediated antigen retrieval using BondTM Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Immunocytochemistry - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)
      Immunocytochemistry - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)
      Immunocytochemistry/ Immunofluorescence analysis of PC-12 (Rat adrenal gland pheochromocytoma) cells labeling Presenilin 2/AD5 with purified ab51249 at 1/50 dilution (5 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Flow Cytometry (Intracellular) - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)
      Flow Cytometry (Intracellular) - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)

      Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Presenilin 2/AD5 with purified ab51249 at 1/50 dilution (5 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

       

    • Immunoprecipitation - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)
      Immunoprecipitation - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)

      ab51249 (purified ) at 1/20 dilution (1ug) immunoprecipitating Presenilin 2/AD5 in HeLa whole cell lysate.
      Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug
      Lane 2 (+): ab51249 & HeLa whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51249 in HeLa whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Immunoprecipitation - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)
      Immunoprecipitation - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)

      Presenilin was immunoprecipitated from 1 mg of HeLa whole cell extract with unpurified ab51249 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab51249 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

      Input: HeLa whole cell extract 10 µg.

       IP (+): ab51249 IP in HeLa whole cell extract.

       IP (-): Rabbit monoclonal IgG (ab172730) instead of ab51249 in HeLa whole cell extract.
      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

       

    • Immunoprecipitation - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)
      Immunoprecipitation - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)

      Presenilin was immunoprecipitated from 1 mg of PC-12 (rat adrenal gland pheochromocytoma) whole cell extract with unpurified ab51249 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab51249 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

      Input: PC-12 whole cell extract 10 µg.

      IP (+): ab51249 IP in PC-12 whole cell extract.

      IP (-): Rabbit monoclonal IgG (ab172730) instead of ab51249 in PC-12 whole cell extract.
      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

       

    文献 (11)


    ab51249 被引用在 11 文献中.

    • Fung S  et al. Early-Onset Familial Alzheimer Disease Variant PSEN2 N141I Heterozygosity is Associated with Altered Microglia Phenotype. J Alzheimers Dis 77:675-688 (2020).PubMed: 32741831
    • Braggin JE  et al. Alternative splicing in a presenilin 2 variant associated with Alzheimer disease. Ann Clin Transl Neurol 6:762-777 (2019).PubMed: 31020001
    • Liu L  et al. A cellular complex of BACE1 and ?-secretase sequentially generates Aß from its full-length precursor. J Cell Biol 218:644-663 (2019).PubMed: 30626721
    • Bissig C  et al. The PIKfyve complex regulates the early melanosome homeostasis required for physiological amyloid formation. J Cell Sci 132:N/A (2019).PubMed: 30709920
    • Naranjo R  et al. Inhibition of the Neuronal Calcium Sensor DREAM Modulates Presenilin-2 Endoproteolysis. Front Mol Neurosci 11:449 (2018).PubMed: 3055964

    客服服务：（早9:00 - 晚19:00）

    027-87561633

    联系我们

    027-87561633

    121545059@qq.com

    武汉东湖新技术开发区高新大道666号光谷生物城生物创新园B1栋b274-9室

    扫描二维码关注我们

    版权所有 ©2018 - 2019 武汉天德生物科技有限公司 鄂ICP备16019xxx号